MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

01-01-2017

Publisher

German Institute for Standardisation (Deutsches Institut für Normung)

National foreword
National Annex NA (informative) - Bibliography
European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Equipment and consumables
7 Sampling
8 Procedure
9 Interpretation of results
10 Expression of results
11 Precision
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation
        of reagents and buffers
Annex C (informative) - Real-time RT-PCR mastermixes
        and cycling parameters
Annex D (informative) - Real-time RT-PCR primers
        and hydrolysis probes for the detection of HAV,
        norovirus GI and GII and mengo virus (process control)
Annex E (informative) - Growth of mengo virus strain
        MC[0]for use as a process control
Annex F (informative) - RNA extraction using
        the NucliSENS[R] system
Annex G (informative) - Generation of dsDNA
        control stocks
Annex H (informative) - Generation of EC RNA stocks
Annex I (informative) - Typical optical plate layout
Annex J (informative) - Method validation studies
        and performance characteristics
Bibliography

Defines a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces.

DevelopmentNote	The 2020 draft amendment version has released for standard DIN EN ISO 15216-1/A1
DocumentType	Standard
Pages	58
PublisherName	German Institute for Standardisation (Deutsches Institut für Normung)
Status	Current
SupersededBy	DIN EN ISO 15216-1:2021-10

Standards	Relationship
EN ISO 15216-1:2017	Identical
ISO 15216-1:2017	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
DIN ISO 5725-2:2002-12	Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method (ISO 5725-2:1994 including Technical Corrigendum 1:2002)
DIN EN ISO 20838 E : 2006	MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF FOOD-BORNE PATHOGENS - REQUIREMENTS FOR AMPLIFICATION AND DETECTION FOR QUALITATIVE METHODS
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
DIN EN ISO 17468 E : 2016	MICROBIOLOGY OF THE FOOD CHAIN - TECHNICAL REQUIREMENTS AND GUIDANCE ON ESTABLISHMENT OR REVISION OF A STANDARDIZED REFERENCE METHOD (ISO 17468:2016)
ISO 17468:2016	Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011	Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions

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DIN EN ISO 15216-1 E : 2017

MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR DETERMINATION OF HEPATITIS A VIRUS AND NOROVIRUS USING REAL-TIME RT-PCR - PART 1: METHOD FOR QUANTIFICATION (ISO 15216-1:2017)

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