Name: EN 14152:2014
Brand: CEN

Foodstuffs - Determination of vitamin B2 by high performance liquid chromatography

Published date

06-04-2014

Publisher

Comite Europeen de Normalisation

Foreword
1 Scope
2 Normative references
3 Principle
4 Reagents
5 Apparatus
6 Procedure
7 Calculation
8 Precision
9 Test report
Annex A (informative) - Examples of HPLC
chromatograms
Annex B (informative) - Precision data
Annex C (informative) - Alternative HPLC Systems
Bibliography

This European Standard specifies a method for the determination of vitamin B2 in food by high performance liquid chromatography (HPLC) and fluorescence detection. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of milk powder and pig's liver ranging from 1,45 mg/100 g to 10,68 mg/100 g. The second study was for the analysis of samples of tube feeding solution, baby food, powdered milk, meal with fruits, yeast, cereal and chocolate powder ranging from 0,21 mg/100 g to 87,1 mg/100 g. Vitamin B2 is the mass fraction of total riboflavin including its phosphorylated derivatives.For further information on the validation, see Clause 8 and Annex B.

Committee	CEN/TC 275
DevelopmentNote	Supersedes PREN 14152. (06/2014)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current
Supersedes	PREN 14152 : DRAFT 2012

Standards	Relationship
NBN EN 14152 : 2014	Identical
NEN EN 14152 : 2014	Identical
NS EN 14152 : 2014	Identical
I.S. EN 14152:2014	Identical
PN EN 14152 : 2014	Identical
SN EN 14152:2014	Identical
UNI EN 14152 : 2014	Identical
UNE-EN 14152:2015	Identical
BS EN 14152:2014	Identical
NF EN 14152 : 2014	Identical
DIN EN 14152:2014-08	Identical
DIN EN 14152:2003-10	Identical

ISO/IEC 17025:2005	General requirements for the competence of testing and calibration laboratories
ISO 3696:1987	Water for analytical laboratory use — Specification and test methods
EN ISO 3696:1995	Water for analytical laboratory use - Specification and test methods (ISO 3696:1987)