Standard Test Method for Immunological Measurement of Antigenic Protein in Hevea Natural Rubber (HNR) and its Products

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

08-06-2023

Publisher

American Society for Testing and Materials

1.1This test method covers an immunological method to determine the amount of antigenic protein in Hevea Natural Rubber and its products using rabbit antisera specific for HNRL proteins. This immunoassay procedure quantitatively measures the level of antigenic latex proteins in solution using an inhibition format. The samples may include glove or other product extracts which have been collected in order to measure the HNR levels. Although this method detects antigenic proteins, it should not be considered as a measure of allergenic proteins. Correlation of protein/antigen levels with the level of allergenic proteins has not been fully established.

1.2For the purpose of this test method, the range of protein will be measured in terms of microgram to milligram quantities.

1.3This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use.

1.4This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Committee	D 11
DocumentType	Test Method
Pages	9
PublisherName	American Society for Testing and Materials
Status	Current
Supersedes	ASTM D 6499 : 2018

ASTM D 7103 : 2019	Standard Guide for Assessment of Medical Gloves
ASTM D 7329 : 2007 : R2018	Standard Specification for Food Preparation and Food Handling (Food Service) Gloves
ASTM D 8243 : 2019	Standard Test Method for Determination of APS Reductase to Estimate Sulfate Reducing Bacterial Bioburdens in Water – Enzyme-Linked Immunosorbent Assay Method
ASTM D 3578 : 2019	Standard Specification for Rubber Examination Gloves
ASTM D 3577 : 2019	Standard Specification for Rubber Surgical Gloves
ASTM D 1076 : 2021	Standard Specification for Rubber—Concentrated, Ammonia Stabilized, Creamed, and Centrifuged Natural Latex
ASTM D 7427 : 2016 : R2021	Standard Test Method for Immunological Measurement of Four Principal Allergenic Proteins (Hev b 1, 3, 5 and 6.02) in Hevea Natural Rubber and Its Products Derived from Latex (Withdrawn 2023)
ASTM D 7850 : 2019	Standard Specification for Biological Properties of Industry Reference Materials (IRM)