CLSI M27 A3 : 3ED 2008

Name: CLSI M27 A3 : 3ED 2008
Brand: CLSI

Withdrawn

REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS

Withdrawn date

23-07-2013

Published date

12-01-2013

Publisher

Clinical Laboratory Standards Institute

Abstract
Committee Membership
Foreword
Updated Information in This Edition
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Antifungal Agents
  6.1 Source
  6.2 Weighing Antifungal Powders
  6.3 Preparing Stock Solutions
  6.4 Number of Concentrations Tested
  6.5 Selection of Antifungal Agents for Routine Testing
       and Reporting
7 Test Procedures
  7.1 Broth Medium
  7.2 Preparing Diluted Antifungal Agents
  7.3 Inoculum Preparation
  7.4 Inoculating RPMI-1640 Medium
  7.5 Incubation
  7.6 Reading Results
  7.7 Interpretation of Results
  7.8 Broth Microdilution Modifications
  7.9 Trailing Growth and the Impact of Time of Reading
  7.10 Other Modifications
8 Quality Control
  8.1 Purpose
  8.2 QC Responsibilities
  8.3 Selecting Reference Strains
  8.4 Storing Reference Strains
  8.5 Routine Use of Reference Strains
  8.6 Batch of Medium and Lot of Plasticware Control
  8.7 QC Frequency
  8.8 Other Control Procedures
  8.9 QC Strains
References
Appendix A. RPMI 1640 Medium
Appendix B. McFarland 0.5 Barium Sulfate Turbidity Standard
Summary of Delegate Comments and Committee Responses
The Quality Management System Approach
Related CLSI Reference Materials

Describes the selection and preparation of antifungal agents; implementation and interpretation of test procedures; and quality control requirements for susceptibility testing of yeasts that cause invasive fungal infections.

DevelopmentNote	Supersedes NCCLS M27 A2. (04/2008) Supplement is available separately, see NCCLS M27 S4. (01/2013)
DocumentType	Miscellaneous Product
PublisherName	Clinical Laboratory Standards Institute
Status	Withdrawn
Supersedes	CLSI M27 A2 : 2ED 2002

CLSI M100 S25 : 25ED 2015	PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS
CLSI EP19 : 2ED 2015	A FRAMEWORK FOR USING CLSI DOCUMENTS TO EVALUATE CLINICAL LABORATORY MEASUREMENT PROCEDURES
EN ISO 16256:2012	Clinical laboratory testing and in vitro diagnostic test systems - Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases (ISO 16256:2012)
CLSI M35 A2 : 2ED 2008	ABBREVIATED IDENTIFICATION OF BACTERIA AND YEAST
CLSI M39 A4 : 4ED 2014	ANALYSIS AND PRESENTATION OF CUMULATIVE ANTIMICROBIAL SUSCEPTIBILITY TEST DATA
DIN EN ISO 16256:2013-04	Clinical laboratory testing and in vitro diagnostic test systems - Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases (ISO 16256:2012)
CLSI M44 A2 : 2ED 2009	METHOD FOR ANTIFUNGAL DISK DIFFUSION SUSCEPTIBILITY TESTING OF YEASTS<br>
UNI EN ISO 16256 : 2013	CLINICAL LABORATORY TESTING AND IN VITRO DIAGNOSTIC TEST SYSTEMS - REFERENCE METHOD FOR TESTING THE IN VITRO ACTIVITY OF ANTIMICROBIAL AGENTS AGAINST YEAST OF FUNGI INVOLVED IN INFECTIOUS DISEASES
I.S. EN ISO 16256:2012	CLINICAL LABORATORY TESTING AND IN VITRO DIAGNOSTIC TEST SYSTEMS - REFERENCE METHOD FOR TESTING THE IN VITRO ACTIVITY OF ANTIMICROBIAL AGENTS AGAINST YEAST OF FUNGI INVOLVED IN INFECTIOUS DISEASES (ISO 16256:2012)
CLSI M7 A10 : 10ED 2015	METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI M57 : 1ED 2016	PRINCIPLES AND PROCEDURES FOR THE DEVELOPMENT OF EPIDEMIOLOGICAL CUTOFF VALUES FOR ANTIFUNGAL SUSCEPTIBILITY TESTING
CLSI M100 S21 : 21ED 2010	PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
CLSI M100 S20 : 20ED 2010	PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
CLSI M100 S19 : 19ED 2008	PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
BS EN ISO 16256:2012	Clinical laboratory testing and in vitro diagnostic test systems. Reference method for testing the in vitro activity of antimicrobial agents against yeast of fungi involved in infectious diseases
ISO 16256:2012	Clinical laboratory testing and in vitro diagnostic test systems Reference method for testing the in vitro activity of antimicrobial agents against yeast fungi involved in infectious diseases
CLSI M100 S24 : 24ED 2014	PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING
CLSI M23 : 5ED 2018	Development of In Vitro Susceptibility Testing Criteria and Quality Control Parameters, 5th Edition
CLSI M59 : 2ED 2018	EPIDEMIOLOGICAL CUTOFF VALUES FOR ANTIFUNGAL SUSCEPTIBILITY TESTING
CLSI M2 A12 : 12ED 2015	PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS
CLSI M27 S4 : 4ED 2012	REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF YEASTS
CLSI M23 A3 : 3ED 2008	DEVELOPMENT OF IN VITRO SUSCEPTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M51 A : 1ED 2010	METHOD FOR ANTIFUNGAL DISK DIFFUSION SUSCEPTIBILITY TESTING OF NONDERMATOPHYTE FILAMENTOUS FUNGI
CLSI M100 S26 : 26ED 2015	PERFORMANCE STANDARDS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING

CLSI M23 A2 : 2ED 2001	DEVELOPMENT OF IN VITRO SUSPECTIBILITY TESTING CRITERIA AND QUALITY CONTROL PARAMETERS
CLSI M7 A7 : 7ED 2006	METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
CLSI M29 A3 : 3ED 2005	ACQUIRED INFECTIONS; APPROVED GUIDELINE
CLSI M24 A : 1ED 2003	SUSCEPTIBILITY TESTING OF MYCOBACTERIA, NOCARDIA, AND OTHER AEROBIC ACTINOMYCETES
CLSI M38 A : 1ED 2002	REFERENCE METHOD FOR BROTH DILUTION ANTIFUNGAL SUSCEPTIBILITY TESTING OF FILAMENTOUS FUNGI
CLSI M11 A7 : 7ED 2007	METHODS FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING OF ANAEROBIC BACTERIA
CLSI M2 A9 : 9ED 2006	PERFORMANCE STANDARDS FOR ANTIMICROBIAL DISK SUSCEPTIBILITY TESTS