CLSI M7 A7 : 7ED 2006
Superseded
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
METHODS FOR DILUTION ANTIMICROBIAL SUSCEPTIBILITY TESTS FOR BACTERIA THAT GROW AEROBICALLY
30-12-2008
12-01-2013
Abstract
Committee Membership
Foreword
Summary of Major Changes in This Document
Subcommittee on Antimicrobial Susceptibility Testing
Mission Statement
1 Scope
2 Introduction
3 Standard Precautions
4 Definitions
5 Indications for Performing Susceptibility Tests
6 Antimicrobial Agents
6.1 Source
6.2 Weighing Antimicrobial Powders
6.3 Preparing Stock Solutions
6.4 Number of Concentrations Tested
7 Selection of Antimicrobial Agents for Routine
Testing and Reporting
7.1 Routine Reports
7.2 Nonproprietary Names
7.3 Selection Guidelines
7.4 Suggested Guidelines for Routine and Selective
Testing and Reporting
8 Inoculum Preparation for Dilution Tests
8.1 Turbidity Standard for Inoculum Preparation
8.2 Direct Colony Suspension Method
8.3 Growth Method
9 Agar Dilution Procedure
9.1 Reagents and Materials
9.2 Preparing Agar Dilution Plates
9.3 Preparing the Inoculum
9.4 Inoculating Agar Dilution Plates
9.5 Incubating Agar Dilution Plates
9.6 Determining Agar Dilution End Points
10 Broth Dilution Procedures (Macrodilution and
Microdilution)
10.1 Mueller-Hinton Broth
10.2 Preparing and Storing Diluted Antimicrobial
Agents
10.3 Broth Dilution Testing
11 Fastidious Organisms
11.1 Haemophilus influenzae and H. parainfluenzae
11.2 Neisseria gonorrhoeae
11.3 Neisseria meningitidis
11.4 Streptococcus pneumoniae and Other
Streptococcus spp
12 Problem Organisms
12.1 Staphylococci
12.2 Enterococci
12.3 Extended-Spectrum, a-Lactamase-Producing,
Gram-Negative Bacilli
13 Inducible Clindamycin Resistance
14 a-Lactamase Tests
14.1 Purpose
14.2 Selecting a a-Lactamase Test
15 Reporting of MIC Results
15.1 Susceptible
15.2 Intermediate
15.3 Resistant
16 Quality Control Procedures
16.1 Purpose
16.2 Quality Control Responsibilities
16.3 Reference Strains for Quality Control
16.4 Storing and Testing Quality Control Strains
16.5 Batch or Lot Quality Control
16.6 MIC Quality Control Limits
16.7 Frequency of Quality Control Testing
16.8 Frequency of Quality Control Testing for
Screening Tests
16.9 Corrective Action
16.10 Reporting Patient Results When Out-of-Control
Tests Occur
16.11 Verification of Patient Test Results
16.12 Other Control Procedures
17 Limitations of Dilution Test Methods
17.1 Application to Various Organism Groups
17.2 Misleading Results
17.3 Emergence of Resistance
18 Screening Tests
References
Appendix A - Quality Control Protocol Flow Charts
Summary of Comments and Subcommittee Responses
Summary of Delegate Comments and Subcommittee Responses
The Quality System Approach
Related CLSI/NCCLS Publications
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