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  • ASTM E 2694 : 2016

    Superseded A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.
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    Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids

    Available format(s):  Hardcopy, PDF

    Superseded date:  05-01-2022

    Language(s):  English

    Published date:  06-10-2016

    Publisher:  American Society for Testing and Materials

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    Abstract - (Show below) - (Hide below)

    1.1 This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

    Scope - (Show below) - (Hide below)

    1.1This test method provides a protocol for capturing, extracting and quantifying the adenosine triphosphate (ATP) content associated with microorganisms found in water-miscible metalworking fluids (MWF).

    1.2The ATP is measured using a bioluminescence enzyme assay, whereby light is generated in amounts proportional to the concentration of ATP in the samples. The light is produced and measured quantitatively as relative light units (RLU) which are converted by comparison with an ATP standard and computation to pg ATP/mL.

    1.3This test method is equally suitable for use in the laboratory or field.

    1.4The test method detects ATP concentrations in the range of 4.0 pg ATP/mL to 400 000 pg ATP/mL.

    1.5Providing interferences can be overcome, bioluminescence is a reliable and proven method for qualifying and quantifying ATP. The method does not differentiate between ATP from different sources, for example, from different types of microorganisms, such as bacteria and fungi.

    1.6The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

    1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

    General Product Information - (Show below) - (Hide below)

    Committee E 34
    Document Type Test Method
    Publisher American Society for Testing and Materials
    Status Superseded
    Superseded By
    Supersedes

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM D 7687 : 2021 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration
    ASTM E 2275 : 2019 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
    ASTM E 979 : 2020 Standard Practice for Evaluation of Antimicrobial Agents as Preservatives for Invert Emulsion and Other Water Containing Hydraulic Fluids
    ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM E 2148 : 2016 Standard Guide for Using Documents Related to Metalworking or Metal Removal Fluid Health and Safety
    ASTM E 2889 : 2012 : R2017 Standard Practice for Control of Respiratory Hazards in the Metal Removal Fluid Environment
    ASTM E 2275 : 2014 Standard Practice for Evaluating Water-Miscible Metalworking Fluid Bioresistance and Antimicrobial Pesticide Performance
    ASTM D 7687 : 2017 Standard Test Method for Measurement of Cellular Adenosine Triphosphate in Fuel and Fuel-associated Water With Sample Concentration by Filtration

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 2523 : 2007 Standard Terminology for Metalworking Fluids and Operations
    ASTM E 1326 : 1998 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 4840 : 1999 : R2018 : EDT 1 Standard Guide for Sample Chain-of-Custody Procedures
    ASTM E 1326 : 2015 Standard Guide for Evaluating Non-culture Microbiological Tests Used for Enumerating Bacteria
    ASTM D 4012 : 1981 : R2009 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 4012 : 2015 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM E 177 : 1990 : REV A : R1996 Standard Practice for Use of the Terms Precision and Bias in ASTM Test Methods
    ASTM D 4840 : 1999 Standard Guide for Sampling Chain-of-Custody Procedures
    ASTM E 1326 : 2015 : REV A Standard Guide for Evaluating Non-culture Microbiological Tests
    ASTM D 1129 : 2003 Standard Terminology Relating to Water
    ASTM D 6161 : 1998 Standard Terminology Used for Crossflow Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
    ASTM D 6161 : 2019 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration, and Reverse Osmosis Membrane Processes
    ASTM D 1129 : 2013 Standard Terminology Relating to Water
    ASTM D 1129 : 2013 : R2020 Standard Terminology Relating to Water
    ASTM E 1497 : 2000 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
    ASTM D 4012 : 1981 : R2002 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM E 2523 : 2013 : R2018 Standard Terminology for Metalworking Fluids and Operations
    ASTM E 1326 : 2006 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 1129 : 2006 Standard Terminology Relating to Water
    ASTM E 691 : 2020 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM E 1497 : 2005 : R2011 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
    ASTM E 1326 : 2008 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
    ASTM D 1129 : 1999 : REV A Standard Terminology Relating to Water
    ASTM E 691 : 2019 : EDT 1 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM D 1129 : 2006 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2003 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2002 Standard Terminology Relating to Water
    ASTM E 1497 : 2005 Standard Practice for Safe Use of Water-Miscible Metal Removal Fluids
    ASTM E 691 : 2009 Standard Practice for Conducting an Interlaboratory Study to Determine the Precision of a Test Method
    ASTM E 2523 : 2006 Standard Terminology for Metalworking Fluids and Operations
    ASTM E 2523 : 2011 Standard Terminology for Metalworking Fluids and Operations
    ASTM D 1129 : 2004 Standard Terminology Relating to Water
    ASTM D 4012 : 1981 : R1997 Standard Test Method for Adenosine Triphosphate (ATP) Content of Microorganisms in Water
    ASTM D 6161 : 2005 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes
    ASTM E 1497 : 2017 Standard Practice for Selection and Safe Use of Water-Miscible and Straight Oil Metal Removal Fluids
    ASTM D 1129 : 2002 : REV A Standard Terminology Relating to Water
    ASTM D 1129 : 2010 Standard Terminology Relating to Water
    ASTM D 6161 : 2010 Standard Terminology Used for Microfiltration, Ultrafiltration, Nanofiltration and Reverse Osmosis Membrane Processes (Withdrawn 2019)
    ASTM D 1129 : 2006 : REV A : EDT 1 Standard Terminology Relating to Water
    ASTM D 1129 : 2001 Standard Terminology Relating to Water
    ASTM D 1129 : 2004 : EDT 1 Standard Terminology Relating to Water
    ASTM E 2523 : 2013 Standard Terminology for Metalworking Fluids and Operations
    ASTM E 1326 : 2013 Standard Guide for Evaluating Nonconventional Microbiological Tests Used for Enumerating Bacteria
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