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PD CEN/TS 15634-2:2012

PD CEN/TS 15634-2:2012

Superseded

Superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

View Superseded by
superseded

A superseded Standard is one, which is fully replaced by another Standard, which is a new edition of the same Standard.

Foodstuffs. Detection of food allergens by molecular biological methods Celery (Apium graveolens). Qualitative determination of a specific DNA sequence in cooked sausages by real-time PCR

Available format(s)

Hardcopy , PDF

Superseded date

11-10-2019

Language(s)

English

Published date

29-02-2012

Publisher

British Standards Institution

Foreword
1 Scope
2 Principle
3 Reagents
4 Apparatus and equipment
5 Analysis steps
6 Validation status and performance criteria
7 Sample type and amounts
8 Limit of detection
9 Interferences
10 Test report
11 Method performance studies
Bibliography

Defines a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).

This Technical Specification specifies a method for the qualitative detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).

Real-time PCR detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082) of celery (Apium graveolens).

The method has been validated on emulsion-type sausages (Bavarian “Leberkäse??) spiked with celery. For this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 °C for 60 min [2].

Committee
AW/275
DocumentType
Standard
Pages
22
PublisherName
British Standards Institution
Status
Superseded
SupersededBy

Standards Relationship
CEN/TS 15634-2 : 2012 Identical

ISO 24276:2006 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
EN ISO 24276:2006/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - GENERAL REQUIREMENTS AND DEFINITIONS (ISO 24276:2006/AMD 1:2013)
EN 15634-1:2009 Foodstuffs - Detection of food allergens by molecular biological methods - Part 1: General considerations

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Hardcopy - English
PDF - English
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