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PD CEN/TS 16707:2014

PD CEN/TS 16707:2014

Current

Current

The latest, up-to-date edition.

Foodstuffs. Methods of analysis for the detection of genetically modified organisms and derived products. Polymerase chain reaction (PCR) based screening strategies

Available format(s)

Hardcopy , PDF

Language(s)

English

Published date

31-10-2014

Publisher

British Standards Institution

Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents
6 Apparatus and equipment
7 PCR analysis
8 PCR method performance criteria
  and validation
Bibliography

Defines screening strategies for the detection of genetically modified (GM) DNA in food products by means of PCR methods.

This Technical Specification describes screening strategies for the detection of genetically modified (GM) DNA in food products by means of PCR methods. The strategies have been established for food matrices, but it can also be applied to other matrices (e.g. feed, seed and samples from field grown plants).

Detection of GM DNA is based on PCR methods targeting segments of transgenic DNA sequences (genetic elements, genetic constructs or insertion sites of transgenes). Various combinations of these PCR methods are involved in screening strategies. The methods are applied simultaneously or hierarchically. The general strategy is based on the matrix approach. Examples for the implementation and application of this approach are described.

In order to ensure reliable analytical results, the document also provides guidelines for the validation of the performance of qualitative PCR methods applied in screening approaches.

Committee
AW/275
DocumentType
Standard
Pages
20
PublisherName
British Standards Institution
Status
Current

Standards Relationship
CEN/TS 16707:2014 Identical

EN ISO 21569 : 2005 AMD 1 2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUALITATIVE NUCLEIC ACID BASED METHODS (ISO 21569:2005/AMD 1:2013)
EN ISO 21570:2005/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - QUANTITATIVE NUCLEIC ACID BASED METHODS (ISO 21570:2005/AMD 1:2013)
EN ISO 21571:2005/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - NUCLEIC ACID EXTRACTION (ISO 21571:2005/AMD 1:2013)
ISO 24276:2006 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products General requirements and definitions
ISO 21571:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Nucleic acid extraction
ISO 21569:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Qualitative nucleic acid based methods
ISO 21570:2005 Foodstuffs Methods of analysis for the detection of genetically modified organisms and derived products Quantitative nucleic acid based methods
EN ISO 24276:2006/A1:2013 FOODSTUFFS - METHODS OF ANALYSIS FOR THE DETECTION OF GENETICALLY MODIFIED ORGANISMS AND DERIVED PRODUCTS - GENERAL REQUIREMENTS AND DEFINITIONS (ISO 24276:2006/AMD 1:2013)

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PDF - English
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