Name: EN 16936:2017
Brand: CEN

Animal feeding stuffs: Methods of sampling and analysis - Screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in compound feed by a microbiological plate test

Published date

05-10-2017

Publisher

Comite Europeen de Normalisation

European foreword
1 Scope
2 Normative references
3 Principle
4 Reagents and materials
5 Apparatus
6 Sample preparation
7 Measurements
8 Determination of concentrations
9 Precision
10 Test report
Annex A (informative) - Preparation of bacterial suspensions
Annex B (informative) - Procedure for the additional
identification of interfering antibiotics
Bibliography

This European Standard presents a method describing the screening on the antibiotics tylosin, virginiamycin, spiramycin, bacitracin-zinc and avoparcin at sub-additive levels in complete feeding stuffs and milk replacers by a microbiological 3-plate test.The limit of detection of the method is 1 mg/kg for avoparcin, tylosin, spiramycin and virginiamycin, and 5 mg/kg for zinc bacitracin. The presence of other (veterinary) antibiotics may interfere with the method.Furthermore, high concentrations of metals (Cu, Zn) may interfere. The method should be used as a qualitative screening method. Positive results can be analysed further by TLC; for confirmatory purposes LC-MS is required [1].A lower limit of detection for zinc bacitracin (3 mg/kg) is achievable (see Table 2), but should be established with an in house validation first.

Committee	CEN/TC 327
DevelopmentNote	Supersedes PREN 16936. (05/2017)
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
NS EN 16936 : 2017	Identical
NEN EN 16936 : 2017	Identical
UNI EN 16936 : 2017	Identical
DIN EN 16936:2016-01 (Draft)	Identical
I.S. EN 16936:2017	Identical
UNE-EN 16936:2017	Identical
DIN EN 16936 E : 2017	Identical
NF EN 16936 : 2017	Identical
SN EN 16936 : 2017	Identical
BS EN 16936:2017	Identical
PN EN 16936 : 2017	Identical
DIN EN 16936:2017-08	Identical

ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO 13969:2003	Milk and milk products — Guidelines for a standardized description of microbial inhibitor tests
EN ISO 13969 : 2004	MILK AND MILK PRODUCTS - GUIDELINES FOR A STANDARDIZED DESCRIPTION OF MICROBIAL INHIBITOR TESTS (ISO 13969:2003)
ISO 4833-1:2013	Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 1: Colony count at 30 °C by the pour plate technique
EN ISO 4833-1:2013	Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 1: Colony count at 30 degrees C by the pour plate technique (ISO 4833-1:2013)
EN ISO 4833-2:2013/AC:2014	MICROBIOLOGY OF THE FOOD CHAIN - HORIZONTAL METHOD FOR THE ENUMERATION OF MICROORGANISMS - PART 2: COLONY COUNT AT 30 DEGREES C BY THE SURFACE PLATING TECHNIQUE (ISO 4833-2:2013/COR 1:2014)
ISO 4833-2:2013	Microbiology of the food chain — Horizontal method for the enumeration of microorganisms — Part 2: Colony count at 30 °C by the surface plating technique
EN ISO 7218 : 2007 COR 2014	MICROBIOLOGY OF FOOD AND ANIMAL FEEDING STUFFS - GENERAL REQUIREMENTS AND GUIDANCE FOR MICROBIOLOGICAL EXAMINATIONS (ISO 7218:2007/AMD 1:2013, CORRECTED VERSION 2014-04-15)