Name: EN ISO 21872-1:2017
Brand: CEN

Microbiology of the food chain - Horizontal method for the determination of Vibrio spp. - Part 1: Detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus (ISO 21872-1:2017)

Amended by

EN ISO 21872-1:2017/A1:2023

Published date

12-07-2017

Publisher

Comite Europeen de Normalisation

European foreword
Foreword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Culture media and reagents
6 Equipment and consumables
7 Sampling
8 Preparation of the test sample
9 Procedure
10 Expression of results
11 Performance characteristics of the method
12 Test report
Annex A (normative) - Diagram of procedure
Annex B (normative) - Composition and preparation
        of the culture media and reagents
Annex C (informative) - Conventional PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin (tdh) and
        thermostable direct related haemolysin (trh)
        genes, Vibrio cholerae and Vibrio vulnificus
Annex D (informative) - Real-time PCR for the
        detection of Vibrio parahaemolyticus,
        thermostable direct haemolysin gene (tdh)
        and Vibrio vulnificus
Annex E (informative) - Results of an interlaboratory
        study
Bibliography

ISO 21872-1:2017 specifies a horizontal method for the detection of enteropathogenic Vibrio spp., which causes human illness in or via the intestinal tract. The species detectable by the methods specified include Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.ISO 21872-1:2017 is applicable to the following:- products intended for human consumption and the feeding of animals;- environmental samples in the area of food production and food handling.

Committee	CEN/TC 463
DocumentType	Standard
PublisherName	Comite Europeen de Normalisation
Status	Current

Standards	Relationship
DIN EN ISO 21872-1:2017-10	Identical
ONORM EN ISO 21872-1 : 2017	Identical
DS EN ISO 21872-1 : 2017	Identical
BS EN ISO 21872-1:2017	Identical
SN EN ISO 21872-1:2017	Identical
ISO 21872-1:2017	Identical
UNI EN ISO 21872-1 : 2017	Identical
I.S. EN ISO 21872-1:2017	Identical
PN EN ISO 21872-1 : 2017	Identical
NEN EN ISO 21872-1 : 2017	Identical
UNE-EN ISO 21872-1:2018	Identical
NF EN ISO 21872-1 : 2017	Identical
DIN EN ISO 21872-1 E : 2017	Identical
SS-EN ISO 21872-1:2017	Identical
NS EN ISO 21872-1 : 2017	Identical
UNI EN ISO 21872-1:2023	Identical

ISO 22174:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions
ISO 5725-1:1994	Accuracy (trueness and precision) of measurement methods and results — Part 1: General principles and definitions
ISO 6887-1:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 1: General rules for the preparation of the initial suspension and decimal dilutions
ISO 7218:2007	Microbiology of food and animal feeding stuffs General requirements and guidance for microbiological examinations
ISO/TS 20836:2005	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Performance testing for thermal cyclers
ISO 20837:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for sample preparation for qualitative detection
ISO 22118:2011	Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics
ISO 20838:2006	Microbiology of food and animal feeding stuffs Polymerase chain reaction (PCR) for the detection of food-borne pathogens Requirements for amplification and detection for qualitative methods
ISO 11133:2014	Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media
ISO 5725-2:1994	Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
ISO 6887-3:2017	Microbiology of the food chain — Preparation of test samples, initial suspension and decimal dilutions for microbiological examination — Part 3: Specific rules for the preparation of fish and fishery products
ISO 17468:2016	Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method
ISO 22119:2011	Microbiology of food and animal feeding stuffs Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens General requirements and definitions