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  • ASTM E 1733 : 2022

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    Standard Guide for Use of Lighting in Laboratory Testing

    Available format(s):  Hardcopy, PDF

    Language(s):  English

    Published date:  12-09-2022

    Publisher:  American Society for Testing and Materials

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    1.1The use of artificial lighting is often required to study the responses of living organisms to contaminants in a controlled manner. Even if the test organism does not require light, the investigator will generally need light to manipulate the samples, and the test might be conducted under the ambient light of the laboratory. One will need to consider not only whether the particular test organism requires light for growth, but also whether the environmental compartment relevant to the test is exposed to light and, if so, what the attributes of light are in that compartment. The light could affect growth of the organism or toxicity of a contaminant, or both. For instance, it has been shown that the toxicity of some organic pollutants is enhanced dramatically by the ultraviolet (UV) radiation present in sunlight (1, 2) .2 Furthermore, the level of ambient lighting in the laboratory (which might affect the test) is not standardized, nor is it comparable to natural environments. It is thus important to consider lighting in all forms of environmental testing. When light is used in the test, one should determine whether the spectral distribution of the radiation source mimics sunlight adequately to be considered environmentally relevant. Also, the container or vessel for the experiment must be transparent, at the point of light entry, to all of the spectral regions in the light source needed for the test.

    1.2It is possible to simulate sunlight with respect to the visible:UV ratio with relatively inexpensive equipment. This guide contains information on the types of artificial light sources that are commonly used in the laboratory, compositions of light sources that mimic the biologically relevant spectral range of sunlight, quantification of irradiance levels of the light sources, determination of spectral outputs of the light sources, transmittance properties of materials used for laboratory containers, calculation of biologically effective radiation, and considerations that should go into designing a relevant light source for a given test.

    1.3Special needs or circumstances will dictate how a given light source is constructed. This is based on the requirements of the test and the environmental compartment to which it is targeted. Using appropriate conditions is most important for any experiment, and it is desirable to standardize these conditions among laboratories. In extreme cases, tests using unusual lighting conditions might render a data set incomparable to other tests.

    1.4The lighting conditions described herein are applicable to tests with most organisms and using most chemicals. With appropriate modifications, these light sources can be used under most laboratory conditions with many types of laboratory vessels.

    1.5The attributes of the light source used in a given study should list the types of lamps used, any screening materials, the light level as an energy fluence rate (in W m−2 ) or photon fluence rate (in μmol m−2 s−1 ), and the transmission properties of the vessels used to hold the test organism(s). If it is relevant to the outcome of a test, the spectral quality of the light source should be measured with a spectroradiometer and the emission spectrum provided graphically for reference.

    1.6The sections of this guide are arranged as follows:



    Referenced Documents




    Summary of Guide


    Significance and Use


    Safety Precautions




     Artificial Lighting


     Light Sources


    Construction of Artificial Light Sources that Mimic Sunlight




     Visible Light


     Visible Light Plus UV-B Radiation


     Simulated Solar Radiation


    Transmission Properties of Lamp Coverings and Laboratory Vessels


     Lamp Coverings


     Laboratory Vessels


    Measurement of Light


     Light Components


     Measurement of Light Quantity




    Biologically Effective Radiation


    Considerations for Designing Light Sources for Environmental Testing


    1.7The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only.

    1.8This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 6.

    1.9This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

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    Committee E 50
    Document Type Guide
    Publisher American Society for Testing and Materials
    Status Current

    Standards Referenced By This Book - (Show below) - (Hide below)

    ASTM E 1963 : 2009 : R2014 Standard Guide for Conducting Terrestrial Plant Toxicity Tests
    ASTM E 1706 : 2020 Standard Test Method for Measuring the Toxicity of Sediment-Associated Contaminants with Freshwater Invertebrates
    ASTM E 1193 : 2020 Standard Guide for Conducting Daphnia magna Life-Cycle Toxicity Tests
    ASTM E 1604 : 2020 Standard Guide for Behavioral Testing in Aquatic Toxicology
    ASTM E 1711 : 2020 Standard Guide for Measurement of Behavior During Fish Toxicity Tests
    ASTM E 1218 : 2021 Standard Guide for Conducting Static Toxicity Tests with Microalgae
    ASTM E 1563 : 2021 : REV A Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid Embryos
    ASTM E 1611 : 2021 Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids
    ASTM D 3978 : 2021 : REV A Standard Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata
    ASTM E 1562 : 2022 Standard Guide for Conducting Acute, Chronic, and Life-Cycle Aquatic Toxicity Tests with Polychaetous Annelids
    ASTM E 1295 : 2022 Standard Guide for Conducting Three-Brood, Renewal Toxicity Tests with Ceriodaphnia dubia
    ASTM E 2591 : 2022 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians
    ASTM E 2455 : 2022 Standard Guide for Conducting Laboratory Toxicity Tests with Freshwater Mussels
    ASTM E 1022 : 2022 Standard Guide for Conducting Bioconcentration Tests with Fishes and Saltwater Bivalve Mollusks
    ASTM E 1241 : 2022 Standard Guide for Conducting Early Life-Stage Toxicity Tests with Fishes

    Standards Referencing This Book - (Show below) - (Hide below)

    ASTM E 1415 : 1991 : R2012 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3 (Withdrawn 2021)
    ASTM E 1415 : 2022 Standard Guide for Conducting Static Toxicity Tests With Lemna gibba G3
    ASTM E 943 : 2008 : R2014 Standard Terminology Relating to Biological Effects and Environmental Fate (Withdrawn 2023)
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